Video 16. Semi in vivo (SIV) procedure for growing pollen tubes.

Time-lapse imaging of Arabidopsis pollen tubes emerging from the cut portion of a pistil, fanning out and traveling across the pollen growth medium. Time elapsed between two frames is indicated in minutes (min) on the top left hand corner.

Reference: Palanivelu, R., and Johnson, M.A. (2010). Functional genomics of pollen tube-pistil interactions in Arabidopsis. Biochem. Soc. Trans. 38 (2): 593–597. Link to article or PubMed citation.